Comparison of Colorimetric and Fluorometric Chemosensors for Protein Concentration Determination and Approaches for Estimation of Their Limits of Detection

Here, we present a direct comparison of different dyes and assays for the determination protein concentrations. We compared classical Bradford assay with two modern based on fluorogenic QuDye ProteOrange showed that reagent achieved excellent results in concentrations as more rivals. also standard approaches determining limit detection (LoD) quantification (LoQ) may not work correctly tested dyes. proposed new approach extends algorithm LoD LoQ determination. This works well both colorimetric dyes, nontrivial fluorescent probes.